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KMID : 0381120140360030293
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Genes and Genomics
2014 Volume.36 No. 3 p.293 ~ p.301
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Three homologous genes encoding functional ?8-sphingolipid desaturase in Populus tomentosa
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Li Shu-Fen
Zhang Guo-Jun
Yuan Ying-Chun
Wang Cong-Hui
Gao Wu-Jun
Deng Chuan-Liang
Lu Long-Dou
Hu Zan-Min
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Abstract
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?8-sphingolipid desaturase is characterized by its ability to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants. No previous studies have been conducted on genes encoding Δ8-sphingolipid desaturases in the woody plant Populus tomentosa. In this study, three genes that encode Δ8-sphingolipid desaturase were isolated from P. tomentosa. Among these genes, PtD8A and PtD8B showed high sequence similarity; whereas PtD8C exhibited large sequence divergence. RT-PCR results showed that PtD8A and PtD8B were expressed in all tissues detected, whereas PtD8C was not expressed in roots. Heterologous expression in yeast revealed that PtD8A/B/C were functional Δ8-sphingolipid desaturases, and can catalyze the C18-phytosphingenine desaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine. However, the conversion rate and ratios of the two products differed. Compared with control cells, transgenic yeasts expressing PtD8A/B/C exhibited enhanced aluminum tolerance. Our findings further elucidated the biochemical functions and evolutionary history of Δ8-sphingolipid desaturases in plants. Candidate genes for breeding new poplar germplasm resources with enhanced tolerance ability to aluminium were also provided.
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KEYWORD
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Biochemical function, ?8-Sphingolipid desaturase, Expression pattern, Evolution, Populus tomentosa
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